KMID : 0380620120440050634
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Korean Journal of Food Science and Technology 2012 Volume.44 No. 5 p.634 ~ p.637
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In vitro Test of Mycelial Growth Inhibition of 5 Fungi Pathogenic to Strawberries by Ultraviolet-C (UV-C) Irradiation
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Kim Seon-Ae
Ahn Soon-Young Oh Wook Yun Hae-Keun
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Abstract
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In strawberry production, among others, the high incidence of diseases by pathogenic fungi resulting in the reduction of fruit yield and quality requires the development of eco-friendly management systems rather than chemical sprays to control them. The diameter of colonies grown in media at 25oC for 5 days was measured to evaluate the in vitro inhibition of mycelial growth of 5 pathogenic fungi by irradiation with ultraviolet (UV-C, 264 nm). The mycelial growth of 5 pathogenic fungi was inhibited in potato dextrose agar (PDA) by the irradiation of UV-C for 1 hour a day, and was dramatically inhibited by the irradiation of UV-C for 9-12 h a day. The irradiation of UV-C for 9-12 h a day inhibited completely the growth of the late blight pathogen, Phytophthora cactorum. The irradiation distance of 40 to 50 cm was effective for the inhibition of mycelial growth of fungi. The mycelial growth of fungi without pre-incubation was inhibited strongly by UV-C irradiation compared to fungi pre-incubated for 2 days without light. The mycelia growth of Colletotrichum gloeosprioides and Fusarium oxysporum was inhibited strongly by UV-C irradiation in vegetable 8 juice agar compared to PDA.
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KEYWORD
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colony, culture media, incubation, mycelial block
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